Ana Witkowski/ Dr. Bostrom Wk 4

 

This week was pretty decent in the city! On Monday, HSS-Cornell CAMEO hosted a trainee specific bone biology teaching retreat. It was a great day of learning/relearning some bone basics and how it can be implemented in the clinic. I think the most interesting thing I learned was how doctors know when to use a plate or a rod on a bone break. Plates are used when the break must reform completely perfect for proper use, as any movement will cause a callous to form. This callous can lead to serious arthritis later in life! The course was a good refresher on basic biology but I learned quite a bit on how the biology informs treatment practices, which was very cool. Very grateful to the organizers and presenters for putting the course together!

The rest of the week has been focused on RNA sequencing gene analysis. I have been trying to modify code from a lab mate in order to get a better picture of how the genes are differentially expressed in our treatment groups in comparison to our controls. I am hoping that changing the heat maps to match ranges and across loaded and controls will help make a bit more sense than the heatmaps currently do! I am also going through and looking up the pathways that are upregulated and downregulated that we found through GSEA analysis and separating them out into a couple major categories, to see if there are any trends in the data that are similar to any previous work in our lab.


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