Andi Garcia-Ortiz/Dr. Carli: Week 7 (July 17th - 21st, 2023)

Monday (July 17th, 2023)

I attended Dr. Carli's clinic on the 7th floor of the Weill Cornell East River Professional Building. Dr. Carli consulted over 20 patients during the clinic with various knee and hip ailments. I left the clinic around 1pm, to attend an animal facility tour at the HSS Research Institute. I have been waiting to take this course for over a month and it was the last training required before I can handle the laboratory mice. I stayed in the lab until 6pm, where I headed over to the HSS OR to shadow a knee revision. The plan was to remove the patient's total knee spacer and insert an articulating spacer. The surgery ended around 11pm and the final treatment plan was the removal of the spacer, addition of antibiotic beads, fasciocutaneous flap, and medial gastric exploration.

Tuesday (July 18th, 2023)

The Prosthetic Joint Infection (PJI) research groups have weekly meetings at 8am at HSS Research Institute on the 6th floor. The group is led by Dr. Mathias Bostrom and Dr. Alberto Carli. After the meeting, I went to Starr 8A-05 in Weill Cornell Medicine to present my summer research project. It was a 4-minute presentation on my cement elution work in the PJI lab. Afterward, I headed back to the HSS Research Institute to start the second round of cement elution studies and collect data for the 1, 2, 4, and 8hr timepoints. We made eight cement coupons and submerged them in 1X PBS for the allotted time points. At every time point, the PBS solution is removed for testing and replaced with new PBS.

Wednesday (July 19th, 2023)

We completed the 24 hr time-point for the second round of cement elutions. We ran the vancomycin colorimetric assay and prepared the MIC assay. We also read the MIC assays from the previous day to determine how effective our vancomycin was at preventing bacterial growth.

Thursday (July 20th, 2023)

We completed the 48 hr time-point for cement elutions. We ran the vancomycin colorimetric assay and prepared the MIC assay. We also read the MIC assays from the previous day to determine how effective our vancomycin was at preventing bacterial growth.

Friday (July 21st, 2023)

We completed the 72 hr time-point for cement elutions. We ran the vancomycin colorimetric assay and prepared the MIC assay. We also read the MIC assays from the previous day to determine how effective our vancomycin was at preventing bacterial growth.

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